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KMID : 0352720080320010073
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Journal of Ginseng Research
2008 Volume.32 No. 1 p.73 ~ p.78
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Inhibitory Activities of Red Ginseng Acidic Polysaccharide in Platelet Aggregation
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Lee Whi-Min
Kamruzzaman S.M.
Song Yong-Bum
Cho Jae-Youl
Park Hwa-Jin
Rhee Man-Hee
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Abstract
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Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng (Panax ginseng C.A. Meyer), has been shown to have a variety of biological functions such as immunostimulating and anti-tumor activities. In the present study, we investigated whether RGAP inhibited ligand-induced platelet aggregation. The washed platelet-rich plasma was prepared from male SD rats with successive centrifugation. The platelets were preincubated with 1 mM of for 2 min either in the presence or in the absence of RGAP and were stimulated with collagen (2.5 ) and thrombin (0.1 U/ml). RGAP dose-dependently inhibited thrombin-induced platelet aggregation with value of . In collagen-induced platelet aggregation, RGAP inhibited the reaction with an value of . RGAP potently suppressed the intracellular calcium ion, which was stimulated by thrombin (0.1 U/ ml). Among mitogen-activated protein kinase (MAPK) subtypes, the extracellular signal-regulated kinase (ERK) 1/2 and p38 MAPK were analyzed in the present study. RGAP inhibited the phosphorylation of ERK2 and p38 MAPK, which was activated by collagen (2.5 ). Finally, these results suggested that besides saponin fraction, RGAP take an important role in the preventive effect of Korean red ginseng against cardiovascular disease such as thrombosis and atherosclerosis.
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KEYWORD
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Red Ginseng Acidic Polysaccharide, Platelet aggregation, Thrombin, Collagen, Extracellular signal-regulated Kinase 1/2, p38 Mitogen-activated protein kinase
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